Sequencing
The Trinity Genome Sequencing Laboratory is located in the Institute of Molecular Medicine (IMM), Trinity College Dublin. The laboratory houses an Illumina Genome Analyzer II (GAII), a second generation DNA sequencing platform that can undertake a range of ultra-high throughput genomics applications. This is a core facility supported by full-time research staff available to all academic and industry-based researchers in Ireland.
June 2009 press release : Minister Lenihan Opens Ireland’s First ‘Next-Generation’ DNA Laboratory at TCD’s Institute of Molecular Medicine
Genome Analyzer Technology
This ultra high-throughput sequencing system enables a wide range of genetic analysis applications including de novo sequencing of novel genomes, resequencing of whole genomes or target regions (e.g. candidate disease genes), transcriptome profiling and the study of DNA regulatory mechanisms. The Genome Analyzer system is powered by Illumina sequencing technology, which uses a massively parallel sequencing-by-synthesis approach to generate billions of bases of high-quality DNA sequence per run.
Applications
DNA Sequencing |
Transcriptome analysis |
Gene Regulation and Control |
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Genome-wide analysis of
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Core Facility
The Trinity Genome Sequencing Laboratory provides the following assistance to researchers interested in undertaking a project using the Illumina Genome Analyzer:
- Advice on study design and costs
- Advice and guidance on sample preparation
- Library preparation, cluster generation, sequencing and primary data analysis
- Advice and guidance on secondary data analysis
For project enquiries/project quotes please contact: Dr Derek Morris or Dr Elaine Kenny
For more information on Illumina Genome Analyzer applications, sample preparation workflow and bioinformatics go to: http://www.illumina.com/sequencing/index.ilmn
For a list of publications using the Illumina Genome Analyzer go to: http://www.illumina.com/publications/pubOnlySeqArticles.ilmn?
For access to Human Genome Sequencing Data generated by the Illumina Genome Analyzer go to: http://www.illumina.com/HumanGenome/?source=sequencing
The Trinity Genome Sequencing Laboratory was established with funding from Science Foundation Ireland through a grant awarded to Dr. Derek Morris.