TrinSeq, the Trinity Genome Sequencing Laboratory is located in the Institute of Molecular Medicine (IMM), Trinity College Dublin. The laboratory now houses an Illumina MiSeq, a benchtop next generation sequencing platform that can undertake a range of ultra-high throughput genomics applications. This is a core facility supported by full-time research staff available to all academic and industry-based researchers in Ireland.
June 2009 press release : Minister Lenihan Opens Ireland’s First ‘Next-Generation’ DNA Laboratory at TCD’s Institute of Molecular Medicine
This ultra high-throughput sequencing system enables a wide range of genetic analysis applications including de novo sequencing of novel genomes, resequencing of whole genomes or target regions (e.g. candidate disease genes), transcriptome profiling and the study of DNA regulatory mechanisms. The MiSeq system is powered by Illumina sequencing technology, which uses a massively parallel sequencing-by-synthesis approach to generate billions of bases of high-quality DNA sequence per run.
Gene Regulation and Control
Genome-wide analysis of
The Trinity Genome Sequencing Laboratory provides the following assistance to researchers interested in undertaking a project using the Illumina MiSeq Benchtop Sequencer:
- Advice on study design and costs
- Advice and guidance on sample library preparation
- Access to automated liquid handling for large scale projects
- Sequencing and primary data analysis
- Advice and guidance on secondary data analysis
How To Get Started
For online information on how to set up your MiSeq sample prep and sequence run: http://support.illumina.com/sequencing/sequencing_instruments/miseq/training.ilmn
For more information on MiSeq reagent kits:
For a list of publications using the Illumina Sequencing Technology go to: http://www.illumina.com/publications/pubOnlySeqArticles.ilmn?
The Trinity Genome Sequencing Laboratory was established with funding from Science Foundation Ireland through a grant awarded to Dr. Derek Morris.